FastQCFastQC Report
Sat 6 Sep 2025
SRR25630409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25630409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43543075
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6925051.5903906648761026No Hit
GTTGGAATTGCGATAATTATTGTTGCTGATGTGAAGTAGGCTCGGGTGTC704800.16186270721578574No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT495030.11368742331587743No Hit
CTTGGTTGAGGCGCCCTGGGACTGCGTCTATTTTTACTCCTAGTGCTGGT470750.10811133572904531No Hit
CGAAGAATCAGAATAAGTGTTGGTAGAGGATTGGGTCTCCTCCGCCTGCC454560.10439317847901189No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)